American Society of Microbiology ASM-ABMM ABMM

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American Society of Microbiology ABMM Sample Questions:

1. A patient develops a severe, rapidly progressive pneumonia with hemoptysis. Gram stain of the sputum shows numerous small, pleomorphic Gram-negative coccobacilli. Growth on Bordet-Gengou agar is observed. The MOST likely causative agent is:

A) Bordetella pertussis
B) Haemophilus influenzae
C) Legionella pneumophila
D) Moraxella catarrhalis

2. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?

A) Whole-genome sequencing (WGS) SNP analysis
B) Pulsed-field gel electrophoresis (PFGE)
C) PCR Ribotyping
D) Multilocus sequence typing (MLST)

3. A patient with a history of recurrent urinary tract infections caused by Escherichia coli develops a new infection. The urine isolate is resistant to trimethoprim-sulfamethoxazole. The MOST common mechanism of resistance to this antibiotic combination in E. coli involves:

A) Mutations in the genes encoding dihydrofolate reductase and dihydropteroate synthase.
B) Expression of efflux pumps that specifically target trimethoprim and sulfamethoxazole.
C) Enzymatic inactivation of trimethoprim and sulfamethoxazole.
D) Increased production of para-aminobenzoic acid (PABA).

4. A clinical microbiology laboratory is implementing a new laboratory information system (LIS). A critical step in the validation process is ensuring the accurate and reliable transfer of data, including patient demographics, test orders, and results. This aspect of validation primarily focuses on:

A) Data integrity and interoperability.
B) Workflow efficiency and user training.
C) Clinical sensitivity and specificity.
D) Analytical sensitivity and specificity.

5. A clinical microbiology laboratory isolates a Gram-negative rod from a wound infection following a dog bite.
The organism grows on MacConkey agar, is oxidase-positive, and produces a characteristic bleach-like odor.
The MOST likely identification is:

A) Bordetella bronchiseptica
B) Pseudomonas aeruginosa
C) Capnocytophaga canimorsus
D) Pasteurella multocida

Solutions: