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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Clinical Chemistry | 20-25% | - Electrolytes, acid-base balance, and blood gases - Carbohydrates, lipids, proteins, and enzymes - Toxicology and liver/kidney function tests - Endocrinology and therapeutic drug monitoring |
| Topic 2: Urinalysis and Other Body Fluids | 5-10% | - Physical, chemical, and microscopic analysis - Urine formation and composition - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing |
| Topic 3: Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Infectious disease serology - Immunologic testing methodologies - Immune system principles and components |
| Topic 4: Blood Banking (Immunohematology) | 15-20% | - Blood components and storage - Blood group systems and antigens - Transfusion reactions and adverse events - Antibody detection and identification - Compatibility testing |
| Topic 5: Laboratory Operations | 5-10% | - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance - Quality control and quality assurance - Pre-analytical, analytical, and post-analytical processes |
| Topic 6: Hematology | 20-25% | - Routine and automated hematology procedures - Hemostasis and coagulation testing - Red blood cell physiology and disorders - White blood cell morphology and diseases |
| Topic 7: Microbiology | 15-20% | - Specimen collection and culture techniques - Mycology, parasitology, and virology - Bacteriology and antimicrobial susceptibility - Identification of pathogenic microorganisms |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. If your reactions are strong at immediate spin (3+) and then get weaker at AHG (w+), it could mean the presence of a strong cold antibody.
Cold antibodies tend to be IgM and their optimum phase for reactivity is immediate spin. Incubation and washing of the sample may cause the agglutination that occurred at room temperature to break down. This would appear as a weaker reaction at AHG.
If the reaction strengths varied in each panel cell then that could be an indication that there are multiple antibodies present.
Your screen cells are 3+ at immediate spin and weak (W)+ at AHG. Your auto control is negative for both phases. Some of your antibody panel cells are 3+ at immediate spin and negative at AHG. What should you suspect?
A) Bad specimen draw
B) A warm autoantibody is present
C) A cold antibody may be present
2. The correct answer which best fits the characteristics in this question is Mycobacterium tuberculosis.
What is the MOST likely identity of an acid-fast bacilli that showed buff colored, dry, heaped colonies; niacin, nitrate, and urease positive; and took 23 days at 35ºC to grow?
A) Mycobacterium bovis
B) Mycobacterium tuberculosis
C) Mycobacterium kansasii
D) Mycobacterium leprae
E) Mycobacterium avium
3. A key laboratory characteristic by which Mycobacterium bovis can be separated from Mycobacterium tuberculosis is:
A) Growth inhibition by thiopine-2-carboxylic acid hydrazide (T2H)
B) Niacin accumulation when grown on egg based medium
C) Semiquantitative catalase
D) Ability to take up iron salts from the culture medium
4. Which EBV markers would be MOST likely positive for an individual who had infectious mononucleosis 9 years ago?
A) All would still be positive
B) Anti-VCA (IgG) and anti-EBNA
C) Anti-VCA (IgM) and heterophile
D) None of the markers would be positive after 9 years
5. The cells are plasma cells. They are larger than normal small lymphocytes with more abundant cytoplasm. The cytoplasm is more basophilic than the cytoplasm of a normal lymphocyte and a well-defined perinuclear halo (clearing in the golgi area) is noticeable. The nucleus is eccentrically placed. Plasma cells are counted in a separate category on a bone marrow differential.
Hematogones are blast-like cells that are more mature than lymphoblasts. They are usually tallied with lymphocytes when performing a bone marrow differential.
What are the cells that are indicated by the red arrows in the image on the right?
A) Hematogones
B) Normal lymphocytes
C) Plasma cells
Solutions:
| Question # 1 Answer: C | Question # 2 Answer: B | Question # 3 Answer: A | Question # 4 Answer: B | Question # 5 Answer: C |


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